Cdc42 GTP 小鼠单抗丨信号分子抗体丨现货速发
更新:2023-03-11 14:37 编号:16910554 发布IP:171.113.47.171 浏览:37次- 发布企业
- 武汉费斯德生物科技有限公司商铺
- 认证
- 资质核验:已通过营业执照认证入驻顺企:第3年主体名称:武汉费斯德生物科技有限公司组织机构代码:91420103MA7H14773E
- 报价
- 人民币¥4800.00元每个
- 品牌
- NewEast Bio
- 货号
- 26905
- 规格
- 30 微升
- 关键词
- Anti-Cdc42-GTP Monoclonal Antibody
- 所在地
- 武汉市江汉区前进五路97号5栋1层8号
- 联系电话
- 027-63497286
- 手机
- 18162422647
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详细介绍
货号: 26905 |
规格: 30 µL |
基因符号: CDC42 |
描述: Anti-Cdc42-GTP Monoclonal Antibody |
背景介绍: Small GTPases are a super-family of cellularsignaling regulators. Cdc42 belongs to the Rho sub-family ofGTPases that regulate cell motility, cell division, and genetranscription. GTP binding increases the activity of Cdc42, and thehydrolysis of GTP to GDP renders it inactive. GTP hydrolysis isaided by GTPase activating proteins (GAPs), while exchange of GDPfor GTP is facilitated by guanine nucleotide exchange factors(GEFs). |
免疫原: Recombinant full length protein of activeCdc42 |
经过测试的应用: IP, IHC |
推荐稀释比: IP: 1 µg for 1~2 mg total cellular proteins IHC, IF: 1:50-1:250 |
浓度: 1 mg/ml |
宿主: Mouse |
形式: Liquid |
克隆性: Monoclonal |
亚型: IgG1 |
纯度: Purified from ascites |
防腐剂: No |
成分: PBS(without Mg2+ andCa2+),pH 7.4, 150 mM NaCl, 50% glycerol |
种属反应性: Anti-Cdc42-GTP monoclonal antibody recognizesactive Cdc42 from vertebrates. |
储存条件: Store at -20°C. Avoid repeated freezing andthawing |
IP-蛋白质印迹: IP/WB analysis of active Cdc42 protein. Purifiedrecombinant Cdc42 proteins were loaded with GDP (lane 1) or GTPγS(lane 2). These proteins were immunoprecipitated withanti-Cdc42-GTP mouse monoclonal antibody (Cat. # 26905). AfterSDS/PAGE, the membrane filter was probed with anti-Cdc42 mousemonoclonal antibody (Cat. # 26008). |
免疫荧光:Immunofluorescentlabeling of Cdc42-GTP in MS1 mouse endothelial cells. The MS1 cellscultured on glass coverslips (Fisher) in DMEM containing 5% FBSwere fixed with 4% paraformaldehyde for 10 minutes at RT (roomtemperature) and permeabilized in PBSN (PBS+0.1%NP40) for 15minutes at RT. The cells were stained with primary antibody(Cdc42-GTP) at 1: 25 dilution in Invitrogen’s CAS-BLOCK (00-8120)reagent at RT for 1 hour. After brief washing in PBSN (3x5min), asecondary Alexa555 conjugated goat anti mouse antibody (InvitrogenA21424) was applied in 1: 200 in CAS-BLOCK at RT for 1 hour. Thecoverslips were then washed in PBSN (3x5min) and mounted usingvectorsheld’s hard set mounting medium (H-1500), and examined usinga Zeiss inverted fluorescence microscope. |
法定代表人 | 梅梅 | ||
注册资本 | 100万 | ||
主营产品 | GTP酶活性抗体,免疫检测试剂盒,EILSA试剂盒,Oncogenes点突变抗体,常规抗体,蛋白,定制服务 | ||
经营范围 | 一般项目:生物化工产品技术研发;工程和技术研究和试验发展;技术服务、技术开发、技术咨询、技术交流、技术转让、技术推广;化工产品销售(不含许可类化工产品);专用化学产品销售(不含危险化学品)(除许可业务外,可自主依法经营法律法规非禁止或限制的项目) | ||
公司简介 | 武汉费斯德生物科技有限公司成立于2022年,坐落于国家生物产业基地——武汉光谷生物城,是一家集科研、生产、销售为一体的生物技术企业,致力于,为广大科研用户提供高质量的生物产品,及一站式的科学技术服务。经营着抗体、蛋白、科研试剂盒等上百种类型产品。可满足生物化学、分子生物学、细胞生物学、免疫学ELISA试剂盒等生物科技实验需求。现客户群体现已覆盖大多科研院校、各大医院、生物公司、制药企业等,我们同时 ... |
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